Histological studies confirmed the gastroprotective activity of D. A significant reduction in the gastric volume, free and total acidity of gastric juice and increase was observed in pH of diclofenac treated group as compared to normal group. The results obtained in this study indicated that the activity of catalase (CAT), peroxidase enzyme (POD), glutathione peroxidase (GSH-Px), glutathione-Stransferase (GST) and glutathione-S-reductase (GSR), glutathione reductase (GSH) and lipid peroxides (TBARS) was significantly decreased and the level of γ-GT and hydrogen peroxide (H2O2) was significantly increased in diclofenac treated group as compared to the control group. There was significant variation in the mean body weight of the treated groups as compared to the control group. The stomach tissues were used for adherent mucus content and also for the histological examination. The gastroprotective effect was assessed from volume of the gastric juice, pH, free and total acidity of the gastric juice, the level of reduced glutathione (GSH), TBARS, H2O2, and activities of enzymic antioxidants catalase (CAT), glutathione peoxidase (GSH-Px), glutathione-Stransferase (GST), γ-Glutamyl transferase (γ-GT) in gastric mucosa. After 12 days of treatment all the rats were sacrificed to collect the serum and gastric content. Group 5 was administered only with 400 mg/kg b.w of DSME. Group 4 was given diclofenac sodium in combination with 400 mg/kg b.w of DSME orally. Group 3 was administered with diclofenac sodium and 200 mg/kg b.w of the DSME. Group 1 was served as control, while group 2 was given 50 mg/kg b.w diclofenac sodium orally once a day for 12 days. These rats were divided in to five groups with six rats in each group. Thirty Sprague dawlay rats of either sex weighing 150-250 g were maintained at the primate facility, Quaid-i- Azam University, Islamabad. The present study was aimed to determine the protective effect of Dalbergia sissoo bark extract (DSME) on diclofenac sodium induced gastric ulcer in experimental rats.
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